Protein method blocking buffers are used to block plates, membranes, and other sample types, and decrease incubation time and background signal for use during enzyme-linked immunosorbent assays (ELISA) and western blot experiments. These products are available in different concentrations and formulations.
TRIS-buffered saline (TBS, 20X), with 1% Tween 20 is used as a wash buffer and for sample preparation in general immunoassay applications, Tween 20 reduces nonspecific binding and protein: protein interactions.TBS is often used for diluting secondary antibodies or streptavidin-AP conjugate.