Bromocresol Green is used in a variety of colorimetric detection technologies. It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC, it is used for visualization of the compounds with functional groups whose pKa is below 5.0. And it is ready-to-use spray and dip reagent for chromatography.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Bromocresol Green is used in a variety of colorimetric detection technologies. It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC, it is used for visualization of the compounds with functional groups whose pKa is below 5.0. And it is ready-to-use spray and dip reagent for chromatography.
Solubility
Soluble in water, ethanol, methyl cellosolve.
Notes
Store away from strong oxidizing agents. Keep container tightly closed. Store in cool, dry conditions in well sealed containers.
RUO – Research Use Only
General References:
- Jabe A. Breland II.; Robert H. Byrne. Spectrophotometric procedures for determination of sea water alkalinity using bromocresol green. Deep Sea Research Part I: Oceanographic Research Papers. 1993, 40, (3), 629-641.
- Erkang Wang.; Zhisheng Sun. Ion transfer of Bromocresol Green across liquid-liquid interfaces. Anal. Chem. 1987, 59, (10), 1414-1417.