Buffer; zwitterionic nondenaturing detergent for solubilizing membrane proteinsCHAPS is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Buffer; zwitterionic nondenaturing detergent for solubilizing membrane proteinsCHAPS is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.
Solubility
Slightly soluble in water.
Notes
Store in a cool. Incompatible with oxidizing agent, strong acid and bases.
RUO – Research Use Only
General References:
- Rodi, P. M.; Gianello, M. D. B.; Corregido, M. C.; Gennaro, A. M. Comparative study of the interaction of CHAPS and Triton X-100 with the erythrocyte membrane. Biochim. Biophys. Acta 2014, 1838 (3), 859-866.
- Ghosh, A.; Saha, R.; Saha, B. Effect of CHAPS and CPC micelles on Ir(III) catalyzed Ce(IV) oxidation of aliphatic alcohols at room temperature and pressure. J. Mol. Liq. 2014, 196, 223-237.