DL-1,4-Dithiothreitol, 99%, for biochemistry

Name: DL-1,4-Dithiothreitol, 99%, for biochemistry
SKU: FSA165685000

1,4-Dithiothreitol, CAS # 3483-12-3, is a reducing agent commonly used to reduce disulphide bonds in protein studies, such as western blot SDS-page electrophoresis. | CAS: 3483-12-03 | C₄H₁₀O₂S₂ | 154.25 g/mol

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Specifications

Chemical Name or MaterialDL-1, 4-Dithiothreitol

CAS3483-12-03

Absorbance(1M soln. in water), (1 M soln. in water) at 0nm, 0.035 max. at 500nm

Health Hazard 1Serious eye damage/eye irritation (category 2), Acute toxicity (category 4), Specific target organ toxicity after single exposure (category 3), Skin corrosion/irritation (category 2)

Health Hazard 2Causes serious eye irritation, Harmful if swallowed, May cause respiratory irritation, May form combustible dust concentrations in air, Causes skin irritation

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General description

1,4-Dithiothreitol (DTT or Cleland's reagent) reduces disulfide bonds in proteins, leading to loss of native conformation
DTT is not stable in solution and should be prepared in small aliquots prior to use; the use of EDTA in the solution can increase the half-life of DTT
Typically used at a final concentration of 0.1M for protein stabilization; recommended to create a 1M stock

Applications

Protects thiol groups from oxidation and acts as a chemical chelator in solution; maintains proteins in the denatured state
Common applications include SDS-page electrophoresis and protein isolation

RUO – Research Use Only