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Guanidine thiocyanate, Molecular Biology Grade
A protein denaturant | CAS: 593-84-0 | CH5N3·CHNS
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Catalog number ALFJ65104.36
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Quantity:
500 g
Specifications
Chemical Name or MaterialGuanidine thiocyanate
CAS593-84-0
Health Hazard 1Skin corrosion/irritation (category 1), Hazardous to the aquatic environment (chronic category 3), Acute toxicity (category 4)
Health Hazard 2Corrosive to the respiratory tract, Causes severe skin burns and eye damage, Contact with acids liberates very toxic gas, Harmful to aquatic life with long lasting effects, Harmful if swallowed, in contact with skin or if inhaled
Melting Point118°C to 122°C
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Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.
Solubility
Soluble in water (1420 g/L) at 20°C, and ethanol (5%).
Notes
Light Sensitive. Keep container tightly closed. Store away from oxidizing agents.
Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.
Solubility
Soluble in water (1420 g/L) at 20°C, and ethanol (5%).
Notes
Light Sensitive. Keep container tightly closed. Store away from oxidizing agents.
RUO – Research Use Only
General References:
- Gregory J. Wadsworth.; Margaret G. Redinbaugh.; John G. Scandalios. A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis . Analytical Biochemistry. 1988, 172, (1), 279-283.
- John M. Chirgwin.; Alan E. Przybyla.; Raymond J. MacDonald.; William J. Rutter. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979, 18, (24), 5294-5299.