Arsenazo III

Arsenazo III (3,6-bis((E)-(2-Arsonophenyl)diazenyl)-4,5-dihydroxynaphthalene-2,7-disulfonic acid, CAS # 1668-00-4) is a metallochrome indicator that is used for detecting calcium levels in plasma and serum. | CAS: 1668-00-4 | C22H18As2N4O14S2 | 776.363 g/mol

Catalog Number	Quantity
ALFJ63031.06	5 g

Catalog number ALFJ63031.06

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Specifications

Chemical Name or MaterialArsenazo III

CAS1668-00-4

Health Hazard 1H301+H331

Health Hazard 2GHS H Statement
H301-H331
Toxic if swallowed.
Toxic if inhaled.

Health Hazard 3P261-P264b-P270-P271-P301+P310-P304+P340-P311-P330-P501c

Suitable for determination of micromolar amounts of calcium

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Arsenazo III is highly specific for calcium and produces absorbance peaks in the 600-660 nm range
• It reacts in slightly acidic conditions, and is soluble in alcohol and semi-soluble in water

Application

• Arsenazo III can detect calcium in permeabilized cells; it is also capable of detecting uranium and thorium
• Arsenazo III has been used in vitro to measure intracellular Ca2+ levels in live neurons alongside patch clamp methods

RUO – Research Use Only

General References:

Yadav R, Pandey BN, Kumar A. Estimation and in-situ detection of thorium in human liver cell culture by arsenazo-III based colorimetric assay. Biometals. 2020 Feb;33(1):75-85. doi: 10.1007/s10534-019-00231-2. Epub 2020 Jan 2. PMID: 31897857.
Hogendoorn C, Roszczenko-Jasińska P, Martinez-Gomez NC, de Graaff J, Grassl P, Pol A, Op den Camp HJM, Daumann LJ. Facile Arsenazo III-Based Assay for Monitoring Rare Earth Element Depletion from Cultivation Media for Methanotrophic and Methylotrophic Bacteria. Appl Environ Microbiol. 2018 Apr 2;84(8):e02887-17. doi: 10.1128/AEM.02887-17. PMID: 29453257; PMCID: PMC5881054.