LDS-sample buffer (4X), reducing is used to prepare protein samples for denaturing gel electrophoresis. It is used in 1D gel electrophoresis, bis-tris gel electrophoresis, tris-acetate gel electrophoresis, protein Gel electrophoresis, proteins, expression, isolation & analysis.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
LDS-sample buffer (4X), reducing is used to prepare protein samples for denaturing gel electrophoresis. It is used in 1D gel electrophoresis, bis-tris gel electrophoresis, tris-acetate gel electrophoresis, protein Gel electrophoresis, proteins, expression, isolation & analysis.
Solubility
Not miscible in water.
Notes
This buffer contains 988mM Tris-HCl, 2.04mM EDTA, 8% LDS, 40% glycerol, 0.88% Coomassie blue, and 0.7mM phenol red, and 8% mercaptoethanol at pH 7.5.
RUO – Research Use Only
General References:
- Sang Woo Seo; Jae-Seong Yang; Inhae Kim; Jina Yang; Byung Eun Min; Sanguk Kim; Gyoo Yeol Jung. Predictive design of mRNA translation initiation region to control prokaryotic translation efficiency. Metabolic Engineering. 2013, 15, 67-74.
- Babak Nazer; b, Soyon Hong; Dennis J. Selkoe. LRP promotes endocytosis and degradation, but not transcytosis, of the amyloid-β peptide in a blood-brain barrier in vitro model. Neurobiology of Disease. 2008, 30, 94-102.