Immuno Clear Standard Modules
Immuno Clear Standard Modules
Actual product may vary
Immuno Clear Standard Modules
Immuno Clear Standard Modules
Immuno Clear Standard Modules

Immuno Clear Standard Modules

Perform applications that deliver well-to-well reproducibility with these immuno clear standard modules. Manufactured to fit standard equipment.
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Catalog NumberSurface TreatmentVolume (Metric) WorkingWell ShapeWell Design
469914MaxiSorp350 μLRound, Flat Bottom (F-well)F16 (2x8 wells)
Catalog number 469914
Price (MYR)
1,692.00
CS
Add to cart
Surface Treatment:
MaxiSorp
Volume (Metric) Working:
350 μL
Well Shape:
Round, Flat Bottom (F-well)
Well Design:
F16 (2x8 wells)
Price (MYR)
1,692.00
CS
Add to cart

Perform applications which are delivering well-to-well reproducibility with Thermo Scientific™ Immuno Clear Standard Modules, which feature Nunc™, Immulon™ and Microtiter™ module plates developed for ELISA techniques. Manufactured to fit standard equipment, modules provide flexible use of components with small sample sizes. They are backed by industry-leading quality standards and certifications.

Features:

  • Several strip formats: Eight-well strip, 12-well strip, 16-well strip (two columns of eight wells)
  • Several well shapes: flat bottom, C bottom (radius edge), U shape wells, StarWells (wells have fins to increase the surface area and enhance performance)
  • Several surfaces to maximize assay performance
  • Modules that remain in frame when inverted
  • Stackable frame marked for lid orientation
  • Polystyrene wells in polyethylene frame
  • Ext. Dim.: 128 × 86mm

Choice of Surface:

  • Thermo Scientific™ PolySorp™, Immulon™ 1B, and Universal Binding (UB) are for adsorption of hydrophobic molecules.
  • Thermo Scientific™ Medisorp™ and Immulon 2HB are slightly hydrophilic and bind a diverse range of biomolecules.
  • Thermo Scientific™ MaxiSorp™, Immulon 4 HBX, and Enhanced Binding(EB) are hydrophilic and ideal for antibody sandwich assays.
  • Thermo Scientific™ MultiSorp™ are very hydrophilic and works well for glycans and water soluble proteins.

Ordering Information:

Modules are freestanding. Cat. No. 95029440 and 464394 are certified. Frames and modules are available separately. Sealing tape and strip caps are available. Color-coded modules are available on request. Contact your customer service or sales representative for further details.

Specifications
BreakapartNo
Binding PropertyHydrophobic
Certifications/ComplianceCertified for adsorption of IgG; CV of <5% between wells
CertifiedYes
FormatStrip Plate
LidWithout Lid
Detection MethodColorimetric
Array2 x 8
Packaging80 Pk., 320 Cases
Binding TypeAdsorption
ColorClear
Product TypeMicroplate Strip
MaterialPolystyrene
Volume (Metric) Well400 μL
No. of Wells16
Volume (Metric) Working350 μL
Surface TreatmentMaxiSorp
Well ShapeRound, Flat Bottom (F-well)
No. per Case320
No. per Pack80
Plate BlockingNone
Quantity320/Cs.
Target MoleculeLipids
Well DesignF16 (2x8 wells)
Unit SizeCS

Frequently asked questions (FAQs)

What frames do you recommend for your Immuno Clear Standard Modules?

We recommend Frames and Accessories for Immuno Breakable Modules, BreakApart frame (Cat. No. 465404). This frame is designed to hold the Immuno Clear Standard Modules.

What length of peptide is ideal for binding to the Thermo Scientific MaxiSorp surface? What are the detection limitations?

We have tested and found that a 3 amino acid peptide (Pro, Leu, Gly) cannot be detected when passively adsorbed on the MaxiSorp surface. However, this peptide can be detected when covalently immobilized using CovaLink NH Modules and CovaLink NH2 Modules and Plates. Using covalent immobilization of small peptide residues, you can typically obtain a better orientation of the molecule and reduced problems with antibody recognition of the peptide due to masking of the epitope. We have discovered that a 7 amino acid peptide from the MHC Class II antigen can be detected when adsorbed on the MaxiSorp surface. We state that the detection limitation using the MaxiSorp surface is between 3 and 7 amino acid residues.
One additional note is that detection is contingent upon the orientation of the peptide when immobilized. If the active site is inactivated or hidden at the site facing the solid phase, no detection signal is observed.

Can I bind either single- or double-stranded DNA to the Thermo Scientific MaxiSorp surface?

Single-stranded DNA can be adsorbed to our MaxiSorp surface using approximately 10 µg ssDNA per mL PBS, pH 8.2, although the stability is uncertain. Based on our experience, ssDNA immobilized on the MaxiSorp surface is so loosely bound that it can be removed by stringent washing.
Double-stranded DNA will not bind to the MaxiSorp surface. DNA, however, can be covalently bound to Nunc NucleoLink Strips.

Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.